8-hydroxyquinoline Copper(WSDTY) and CuCl2 were dissolved in DMSO to a final concentration of 50 mM. Then CuCl2 was mixed with each in a 1:1 ratio and qualitatively examined for color change and precipitate formation. After mixing, solutions were heated and vortexed repeatedly until clear. For the visual studies, solutions were examined for color change and precipitation as indicators of complex formation. In cellular studies, however, stock concentrations were kept lower (10 and 20 mM) prior to dilution during mixing in order to prevent precipitation.
The MTT assay was used to determine the effects of these agents on overall proliferation of cells. Cells were plated in a 96-well plate and grown to 70–80% confluency, followed by addition of each compound at an indicated concentration for 24 h. MTT (1 mg/ml) in PBS was then added to wells and incubated at 37°C for 4 h to allow for complete cleavage of the tetrazolium salt by metabolically active cells. Next, MTT was removed and 100 μl of DMSO was added, followed by colorimetric analysis using a multilabel plate reader at 560 nm. Absorbance values plotted are the mean from triplicate experiments.
Wujiang Weishida Copper Technology Co.,Ltd. has 12,000 square meters of production base. By virtue of their sincere cooperation, reciprocity of the business philosophy for the industry users around the country to provide high-quality non-ferrous metal products.Weishida specializes in producing cuprous chloride, basic copper carbonate, copper acetate, copper oxide, copper sulfate. Our company has established a perfect quality assurance system, quality inspection and testing equipment, testing, testing and technology development strength, to ensure the stability of product quality, fully able to meet the pre-market after-sales service to provide users with the need.
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The MTT assay was used to determine the effects of these agents on overall proliferation of cells. Cells were plated in a 96-well plate and grown to 70–80% confluency, followed by addition of each compound at an indicated concentration for 24 h. MTT (1 mg/ml) in PBS was then added to wells and incubated at 37°C for 4 h to allow for complete cleavage of the tetrazolium salt by metabolically active cells. Next, MTT was removed and 100 μl of DMSO was added, followed by colorimetric analysis using a multilabel plate reader at 560 nm. Absorbance values plotted are the mean from triplicate experiments.
Wujiang Weishida Copper Technology Co.,Ltd. has 12,000 square meters of production base. By virtue of their sincere cooperation, reciprocity of the business philosophy for the industry users around the country to provide high-quality non-ferrous metal products.Weishida specializes in producing cuprous chloride, basic copper carbonate, copper acetate, copper oxide, copper sulfate. Our company has established a perfect quality assurance system, quality inspection and testing equipment, testing, testing and technology development strength, to ensure the stability of product quality, fully able to meet the pre-market after-sales service to provide users with the need.
Click Cuprous Chloride to learn about more information
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