Several chemical tests utilize Basic Copper Sulphate(WSDTY). It is used in Fehling’s solution and Benedict’s solution to test for reducing sugars, which reduce the soluble blue copper(II) sulfate to insoluble red copper(I) oxide. Copper(II) sulfate is also used in the Biuret reagent to test for proteins.

Basic Copper Sulphate is used to test blood for anemia. The blood is tested by dropping it into a solution of copper sulfate of known specific gravity – blood which contains sufficient hemoglobin sinks rapidly due to its density, whereas blood which does not sink or sinks slowly has insufficient amount of hemoglobin.

In a flame test, its copper ions emit a deep green light, a much deeper green than the flame test for barium.Copper sulfate is employed at a limited level in organic synthesis.The anhydrous salt is used as a dehydrating agent for forming and manipulating acetal groups. The hydrated salt can be intimately mingled with potassium permanganate to give an oxidant for the conversion of primary alcohols.

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